A case report: Electrochemical impedance spectroscopy as an Al-ternative for cell counting chambers of yeast (Saccharomyces cerevisiae) for brewery applications

Advanced technologies, such as electrochemical impedance spectroscopy (EIS), are a valuable tool which can enhance and simplify the industrial process monitoring if used correctly. State-of-the-art approaches for screening cell growth of example yeast during brewing still heavily rely on offline methods methylene blue or florescence dye-based staining, and/or usage flow cytometric measurements. These methods, while being accurate, very time consuming require heavy manual effort. Furthermore, span needed to obtain counting result lead time-delayed response signal impact quality final product. In recent studies, applications low-frequency EIS in α-regime were determination counts metabolic state Saccharomyces cerevisiae. This method has proven be reliable also shown high potential scale applications. The online biomass monitoring, well viable count, feasibility study was performed in-house at Stiegl Brewery Salzburg/Austria founded 1492.